| Expression pattern: |
UP |
| Associated gene: |
Receptor tyrosine kinase-like orphan receptor 1 (ROR1), phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha (PI3KCA) |
| Associated microRNA: |
miR-27b-3p |
| Biological function: |
promotes GC cell proliferation, migration and invasion; suppresses apoptosis; inhibits autophagy machinery activation |
| Molecular mechanism: |
hsa_circ_0006470 acts as a ceRNA sponge for miR-27b-3p, regulating the miR-27b-3p/ROR1 axis and the miR-27b-3p/PI3KCA axis to promote malignant phenotypes and repress autophagy in GC cells. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); autophagy (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RT-qPCR; FISH / smFISH; Luciferase Reporter Assay; Bioinformatics Analysis; Transfection; CCK8; Transwell Assay; Western Blot; IF (Immunofluorescence) |
| Clinical significance: |
could be explored as new targets for GC diagnosis and treatment mediated by the non-coding RNAs and epigenetic regulation |
| Description: |
hsa_circ_0006470 is up-regulated in gastric cancer cell lines and acts as an oncogenic circRNA. It sponges miR-27b-3p to regulate ROR1 and PI3KCA, thereby promoting proliferation, migration and invasion while suppressing autophagy in GC cells. |
| Confidence score: |
0.6722 |