| Expression pattern: |
UP |
| Associated gene: |
CD39 / ENTPD1, AGO2 |
| Associated microRNA: |
miR-661, miR-671-5p |
| Biological function: |
promotes proliferation, migration, and invasion of gastric cancer cells; facilitates CD8+ T cell exhaustion; promotes anti-PD-1 resistance |
| Molecular mechanism: |
ceRNA mechanism: circ_0001947 sponges miR-661 and miR-671-5p to increase CD39 (ENTPD1), leading to CD8+ T cell exhaustion and anti-PD-1 resistance; transmitted via sEVs |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); immune regulation (promotes); T cell exhaustion (promotes); drug resistance (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Sanger Sequencing; FISH / smFISH; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Flow Cytometry（Non-apoptosis/cycle）; Clinical Sample Validation; Cohort Study; Survival Analysis; Bioinformatics Analysis; Western Blot |
| Clinical significance: |
circ_0001947 was associated with poor clinical outcome and correlated with poor prognosis for GC patients |
| Description: |
circ_0001947 (circAFF2) is up-regulated in gastric cancer and enriched in GC-derived sEVs. It promotes tumor cell proliferation/migration/invasion and drives CD8+ T cell exhaustion and anti-PD-1 resistance by sponging miR-661/miR-671-5p to increase CD39 (ENTPD1). |
| Confidence score: |
0.8823 |