| Expression pattern: |
UP |
| Associated gene: |
BRCA1 |
| Associated microRNA: |
miR-498 |
| Biological function: |
Breast cancer biomarker with diagnostic potential; upregulated in breast cancer patients; regulates BRCA1 by binding to miR-498 through a ceRNA mechanism. |
| Molecular mechanism: |
circROBO1 regulated BRCA1 by binding to miR-498 through ceRNA mechanism. |
| Biological pathway or process: |
ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
circROBO1 showed diagnostic potential for breast cancer detection; ROC analysis in PCR products from healthy donors and breast cancer patients showed AUC = 0.875. |
| Description: |
This study established a CRISPR-Cas13a/Cas12a fluorescence system for sensitive detection of circROBO1 together with BRCA1 in breast cancer-related samples. circROBO1 was upregulated in breast cancer patients compared with healthy donors and showed diagnostic potential with an ROC AUC of 0.875. Mechanistically, the study reported a ceRNA relationship in which circROBO1 regulates BRCA1 by binding miR-498. |
| Confidence score: |
0.459 |