| Expression pattern: |
UP |
| Associated gene: |
AFP, Ago2, exosomes |
| Associated microRNA: |
miR-1270 |
| Biological function: |
promotes proliferation; promotes migration; promotes tumor growth; promotes metastasis; promotes malignant behaviors of surrounding normal cells |
| Molecular mechanism: |
Acts as a ceRNA by sponging miR-1270 to upregulate AFP; transferred via exosomes to recipient cells to mediate phenotypic changes |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); metastasis (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Sanger Sequencing; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; FISH / smFISH; Clinical Sample Validation; Transfection; CCK8; EdU Staining; Transwell Assay; Western Blot; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Bioinformatics Analysis; Survival Analysis |
| Clinical significance: |
Low expression of circRNA Cdr1as in the tumor tissues was associated with tumor diameter, serum alpha-fetoprotein (AFP) and tumor satellite. |
| Description: |
CircRNA Cdr1as is up-regulated in HCC tissues and cell lines and promotes proliferation, migration, tumor growth and lung metastasis. It mainly localizes to the cytoplasm and functions as a ceRNA that sponges miR-1270 to increase AFP expression. Cdr1as is enriched in exosomes and can be transferred to recipient cells, enhancing proliferative and migratory phenotypes. |
| Confidence score: |
0.8657 |