| Expression pattern: |
UP |
| Associated gene: |
PPP1CA, UBA1, YAP, TEAD1-4, 14-3-3 |
| Associated microRNA: |
- |
| Biological function: |
promotes CRC cell proliferation, migration, invasion, and metastasis |
| Molecular mechanism: |
CircGPRC5A binds PPP1CA, inhibits UBA1-PPP1CA binding to prevent PPP1CA ubiquitin-mediated proteasomal degradation, thereby increasing PPP1CA-dependent dephosphorylation of YAP (Ser127/Ser109) and promoting YAP nuclear activity |
| Biological pathway or process: |
Hippo/YAP (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); ubiquitination (inhibits) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Microarray; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RNase R Treatment; Sanger Sequencing; Back-Splice Junction PCR / divergent primers PCR; Actinomycin D / DRB Stability Assay; Northern Blot; RNA Pull-Down; RIP (RNA Immunoprecipitation); IF (Immunofluorescence); Co-IP; Western Blot; Bioinformatics Analysis; Transfection; CCK8; Colony Formation Assay; EdU Staining; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry) |
| Clinical significance: |
High circGPRC5A tissue expression was correlated with tumor size, lymph node status, T stage, and TNM stage. |
| Description: |
CircGPRC5A is upregulated in colorectal cancer and promotes proliferation, migration/invasion, and metastasis. It binds PPP1CA and blocks UBA1-PPP1CA interaction to reduce PPP1CA ubiquitin-mediated proteasomal degradation, increasing PPP1CA protein and driving YAP dephosphorylation (Ser127/Ser109) and pro-tumor YAP nuclear activity. |
| Confidence score: |
0.8585 |