| Expression pattern: |
UP |
| Associated gene: |
IRS2 |
| Associated microRNA: |
miR-338-3p |
| Biological function: |
Promotes proliferation, migration, invasion, metastasis and tumor growth, and inhibits apoptosis of NSCLC cells. |
| Molecular mechanism: |
circ_0000003 acts as an endogenous competitive RNA sponge for miR-338-3p and positively regulates IRS2 expression. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Transfection; CCK8; BrdU; Annexin V/PI Flow Cytometry; Western Blot; Transwell Assay; Luciferase Reporter Assay; In Vivo Animal Model |
| Clinical significance: |
High circ_0000003 expression was correlated with local lymph node metastasis, higher TNM staging/T stage, poor differentiation, and was expected to be an indicator of poor prognosis of NSCLC. |
| Description: |
circ_0000003 is up-regulated in NSCLC tissues and cell lines and is associated with adverse clinicopathological features, including lymph node metastasis and higher TNM/T stage. Functionally, circ_0000003 promotes NSCLC cell proliferation, migration, invasion, metastasis and xenograft tumor growth while suppressing apoptosis. Mechanistically, it acts as a ceRNA for miR-338-3p to increase IRS2 expression through the circ_0000003/miR-338-3p/IRS2 axis. |
| Confidence score: |
0.6542 |