| Expression pattern: |
UP |
| Associated gene: |
HK2, Bax, Bak, Bcl-2, Ki67 |
| Associated microRNA: |
miR-512-3p |
| Biological function: |
Circ_0087862 promotes CRC cell viability, proliferation, migration, invasion, glycolysis and xenograft tumor growth, and inhibits apoptosis. |
| Molecular mechanism: |
Circ_0087862 acts as a miR-512-3p sponge to positively regulate HK2 expression, forming a circ_0087862/miR-512-3p/HK2 axis in CRC progression. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); glycolysis (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Microarray; MTT; Colony Formation Assay; EdU Staining; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; IHC (Immunohistochemistry); In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
Circ_0087862 is suggested as a promising tumor promoter and may provide a new light for CRC therapy. |
| Description: |
Hsa_circ_0087862 is up-regulated in colorectal cancer tissues and cell lines and functions as a tumor-promoting circRNA. Its knockdown suppresses CRC cell proliferation, migration, invasion, glycolysis and xenograft tumor growth while promoting apoptosis. Mechanistically, circ_0087862 sponges miR-512-3p to up-regulate HK2 expression. |
| Confidence score: |
0.8256 |