circRNA basic information
circBase ID: hsa_circ_0076305
Name: hsa_circ_PGC
Synonym: -
Host Gene: PGC
Genomic location(hg19): chr6:41704448-41712252:-
Genomic location(hg38): chr6:41736710-41744514:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer
Disease DO ID:
3908
Disease MeSH ID:
D002289
Disease NCIt ID:
C2926
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissues; adjacent normal tissues

Cell lines:

H460; PC9; BEAS-2B

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: FOXR2, beta-catenin, c-Myc, HK-2, MMP9, E-cad
Associated microRNA: miR-532-3p
Biological function: Circ-PGC functions as an oncogene in NSCLC, promoting cell viability, colony formation, migration, invasion, glycolysis metabolism and tumor growth, while inhibiting apoptosis.
Molecular mechanism: Circ-PGC acts as a ceRNA/molecular sponge for miR-532-3p to positively regulate FOXR2 expression, activating the Wnt/beta-catenin signaling pathway.
Biological pathway or process:

Wnt/beta-catenin (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); glycolysis (promotes); ceRNA regulation (other)

Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Wound Healing Assay; Transwell Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Western Blot; In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis

Clinical significance:

High circ-PGC expression was associated with poor overall survival of NSCLC patients within 5 years.

Description:

Circ-PGC is up-regulated in NSCLC tissues and cell lines and high expression is associated with poor overall survival. Functionally, circ-PGC promotes NSCLC cell proliferation, migration, invasion, glycolysis and in vivo tumor growth, while suppressing apoptosis. Mechanistically, circ-PGC sponges miR-532-3p to increase FOXR2 expression and may activate Wnt/beta-catenin signaling.

Confidence score:

0.8175

Other information
Title:

Circ-PGC increases the expression of FOXR2 by targeting miR-532-3p to promote the development of non-small cell lung cancer.

Journal: Cell cycle (Georgetown, Tex.)
Published: 2021
PubMed ID: 34494941
Study type:

combined biological and clinical study

Data availability: available from the corresponding author on reasonable request
Code availability: -