| Expression pattern: |
UP |
| Associated gene: |
HDAC4, HMGB1, AGO2 |
| Associated microRNA: |
miR-141-3p |
| Biological function: |
promotes proliferation, migration and invasion of osteosarcoma cells |
| Molecular mechanism: |
circ-LRP6 acts as a sponge for miR-141-3p, thereby relieving miR-141-3p-mediated repression of HDAC4 and HMGB1 |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Clinical Sample Validation; Transfection; CCK8; Transwell Assay; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Western Blot; Survival Analysis; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circ-LRP6 expression is associated with advanced clinical stage, lung metastases, lower overall survival, and has diagnostic value (AUC=0.6960). |
| Description: |
circ-LRP6 is up-regulated in osteosarcoma tissues and cell lines and functions as an oncogenic circRNA. It sponges miR-141-3p to increase HDAC4 and HMGB1, thereby promoting osteosarcoma cell proliferation, migration, and invasion; high circ-LRP6 is associated with worse overall survival and shows diagnostic potential. |
| Confidence score: |
0.8168 |