| Expression pattern: |
UP |
| Associated gene: |
AGO2, TNKS, beta-catenin, CCND1, AXIN2 |
| Associated microRNA: |
miR-149-5p, miR-331-3p |
| Biological function: |
Promotes colorectal cancer progression by enhancing proliferation, cell cycle progression, migration and invasion; promotes tumor growth in vivo. |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge for miR-149-5p, relieving repression of TNKS, thereby activating Wnt/beta-catenin signaling (AXIN2 degradation, increased nuclear beta-catenin and CCND1). |
| Biological pathway or process: |
proliferation (promotes); cell cycle (promotes); migration (promotes); invasion (promotes); Wnt/beta-catenin (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RNase R Treatment; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; ISH (In Situ Hybridization); RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; RNA-seq; Bioinformatics Analysis; Transfection; EdU Staining; Colony Formation Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; IF (Immunofluorescence); IHC (Immunohistochemistry); In Vivo Animal Model; Clinical Sample Validation; Cohort Study; Survival Analysis |
| Clinical significance: |
High circ5615 expression is correlated with higher T stage and is an independent prognostic factor associated with shorter overall survival in CRC patients. |
| Description: |
circ5615 (hsa_circ_0005615), derived from NFATC3, is upregulated in colorectal cancer and mainly localizes to the cytoplasm. It promotes CRC proliferation/cell-cycle progression and tumor growth by sponging miR-149-5p to de-repress TNKS, thereby activating the Wnt/beta-catenin pathway (e.g., increased nuclear beta-catenin and CCND1). High circ5615 expression correlates with higher T stage and poor overall survival. |
| Confidence score: |
0.8991 |