| Expression pattern: |
UP |
| Associated gene: |
CDC25A, CCNB1 |
| Associated microRNA: |
hsa-miR-497-5p |
| Biological function: |
Promotes gastric cancer cell cycle progression and proliferation, and inhibits apoptosis; knockdown suppresses tumor growth in vivo. |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge: hsa_circRNA_104433 binds hsa-miR-497-5p, relieving suppression of CDC25A (and downstream cell-cycle factors such as CCNB1). |
| Biological pathway or process: |
cell cycle (promotes); proliferation (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; Cell Cycle Assay; In Vivo Animal Model; Western Blot |
| Clinical significance: |
Its expression was positively correlated with the degree of differentiation and the size of the tumor; proposed as a potential therapeutic target and biomarker for GC. |
| Description: |
hsa_circRNA_104433 is up-regulated in gastric cancer and promotes tumor cell cycle progression and proliferation while inhibiting apoptosis. Mechanistically, it binds (sponges) hsa-miR-497-5p to increase CDC25A (and cell-cycle proteins such as CCNB1), and its knockdown suppresses xenograft tumor growth. |
| Confidence score: |
0.6829 |