circRNA basic information
circBase ID: -
Name: -
Synonym: Circ_100565
Host Gene: -
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tissues; adjacent normal tissues; tumor
Cell lines:

Calu-3; Calu-6; A549; H1299; HBE1
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: HMGA2, Argonaute2
Associated microRNA: miR-506-3p
Biological function: promotes proliferation, migration and invasion; promotes tumor growth; promotes EMT
Molecular mechanism: ceRNA mechanism: circ_100565 sponges miR-506-3p to upregulate HMGA2
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); ceRNA regulation (promotes)
Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Colony Formation Assay; Cell Cycle Assay; Transwell Assay; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; In Vivo Animal Model; Survival Analysis; Clinical Sample Validation
Clinical significance:

high circ_100565 expression was positively associated with poor overall survival of NSCLC patients; positively correlated with lymph node metastasis and TNM stages
Description:

circ_100565 is up-regulated in NSCLC and mainly localized in the cytoplasm. It promotes NSCLC proliferation, migration, invasion, EMT, and tumor growth by acting as a miRNA sponge for miR-506-3p, thereby relieving repression of HMGA2. High circ_100565 expression is associated with poorer overall survival and more aggressive clinicopathologic features.
Confidence score:

0.8279
Other information
Title:

Circ_100565 promotes proliferation, migration and invasion in non-small cell lung cancer through upregulating HMGA2 via sponging miR-506-3p.

Journal: Cancer cell international
Published: 2020
PubMed ID: 32425695
Study type:

combined biological and clinical study
Data availability: All data generated or analysed during this study are included in this published article.
Code availability: -