| Expression pattern: |
DN |
| Associated gene: |
- |
| Associated microRNA: |
- |
| Biological function: |
Underexpressed in RR-MS peripheral blood and proposed as a blood biomarker for multiple sclerosis; no disease functional assays were reported. |
| Molecular mechanism: |
Bioinformatic analysis suggested shared miRNA targeting with circ_0005402 and possible cooperative regulation of these miRNAs; possible parental gene expression regulation or RBP interaction was discussed but not experimentally confirmed. |
| Biological pathway or process: |
ceRNA regulation (other); other pathway/process (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Microarray; Clinical Sample Validation; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
Potential blood biomarker for RR-MS; ROC AUC 0.706 with sensitivity 55% and specificity 88.9%. |
| Description: |
circ_0035560, renamed circ_0003452_2 by the authors after Sanger sequencing revealed a different junction structure, is a human ANXA2-derived circRNA significantly downregulated in RR-MS blood. It was proposed as a new biomarker for multiple sclerosis, with ROC AUC 0.706, and bioinformatic analysis suggested shared miRNA regulation with circ_0005402. |
| Confidence score: |
0.6841 |