circRNA basic information
circBase ID: hsa_circ_0007534
Name: hsa_circ_DDX42
Synonym: -
Host Gene: DDX42
Genomic location(hg19): chr17:61869771-61877977:+
Genomic location(hg38): chr17:63792411-63800617:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer / NSCLC
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tissues; adjacent non-tumor tissue samples; non-tumorous samples; subcutaneous transplantation tumor
Cell lines:

A549; H358; H1581; H1975; H1299; 16HBE
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: -
Associated microRNA: -
Biological function: Promotes NSCLC cell growth, proliferation, clonogenic capacity, migration, invasion, EMT and xenograft tumor growth; inhibits apoptosis.
Molecular mechanism: hsa_circ_0007534 exerts oncogenic properties partly by suppressing caspase-3 and caspase-9-associated apoptosis and by affecting the EMT pathway, including regulation of E-cadherin, Snail, N-cadherin and Vimentin protein levels.
Biological pathway or process:

proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Cohort Study; Survival Analysis
Clinical significance:

Up-regulation is linked to lymph node invasion and advanced TNM stage; high expression predicts shorter survival and serves as an independent unfavorable prognostic predictor for 5-year overall survival in NSCLC patients.
Description:

hsa_circ_0007534 is up-regulated in NSCLC tissues and cell lines and its high expression is associated with lymph node invasion, advanced TNM stage and poor overall survival. Functionally, it promotes NSCLC cell proliferation, colony formation, migration, invasion, EMT and xenograft tumor growth while inhibiting apoptosis, suggesting an oncogenic and prognostic role.
Confidence score:

0.6742
Other information
Title:

Upregulation of circular RNA hsa_circ_0007534 predicts unfavorable prognosis for NSCLC and exerts oncogenic properties in vitro and in vivo.

Journal: Gene
Published: 2018
PubMed ID: 30017736
Study type:

combined biological and clinical study
Data availability: -
Code availability: -