| Expression pattern: |
UP |
| Associated gene: |
ErBB3 |
| Associated microRNA: |
miR-205-5p |
| Biological function: |
Promotes PCa cell proliferation, relieves SC-induced cell apoptosis and G0/G1 phase arrest, and mediates SC-induced anti-proliferation and pro-apoptosis effects. |
| Molecular mechanism: |
circHMGCS1 acts as a sponge for miR-205-5p, thereby regulating ErBB3 expression and PI3K-AKT signaling; SC inhibits circHMGCS1 expression, reducing circHMGCS1-miR-205-5p binding and leading to ErBB3 downregulation, PI3K-AKT pathway inactivation, G0/G1 arrest, and apoptosis. |
| Biological pathway or process: |
PI3K/AKT (promotes); proliferation (promotes); apoptosis (inhibits); cell cycle (promotes); ceRNA regulation (promotes); drug resistance (other) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; Microarray; FISH / smFISH; RNA Pull-Down; Luciferase Reporter Assay; Transfection; MTT; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; IF (Immunofluorescence); Western Blot; In Vivo Animal Model; TUNEL; IHC (Immunohistochemistry); Bioinformatics Analysis |
| Clinical significance: |
circHMGCS1 is highly expressed in PCa, positively correlated with malignancy, and may be a novel therapeutic target for prostate cancer treatment. |
| Description: |
circHMGCS1 is up-regulated in prostate cancer and is associated with higher malignancy. It promotes PCa cell proliferation and counteracts Sanggenon C-induced apoptosis and G0/G1 arrest by sponging miR-205-5p, thereby maintaining ErBB3 expression and activating PI3K/AKT signaling. SC exerts anti-tumor effects by suppressing circHMGCS1, which releases miR-205-5p to downregulate ErBB3 and induce cell-cycle arrest and apoptosis. |
| Confidence score: |
0.8207 |