| Expression pattern: |
UP |
| Associated gene: |
TRAF6, MAPK11, Ago2 |
| Associated microRNA: |
miR-516a-5p, miR-145-5p |
| Biological function: |
Promotes HCC cell proliferation and tumor growth (tumorigenesis) in vitro and in vivo. |
| Molecular mechanism: |
Acts as a miRNA sponge (ceRNA) for miR-516a-5p (and miR-145-5p), thereby derepressing miR-516a-5p targets TRAF6 and MAPK11; involves Ago2/RISC. |
| Biological pathway or process: |
proliferation (promotes); MAPK (other); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; RT-qPCR; divergent primers PCR; RNase R Treatment; Transfection; MTT; Colony Formation Assay; In Vivo Animal Model; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Western Blot; Clinical Sample Validation |
| Clinical significance: |
Serum circ_0001955 is higher in HCC patients than healthy controls and decreases after surgery. |
| Description: |
circ_0001955 is upregulated in HCC tissues and patient serum and functions as an oncogenic circRNA. It sponges miR-516a-5p (also predicted/implicated with miR-145-5p), thereby relieving repression of TRAF6 and MAPK11 and promoting HCC cell proliferation and xenograft tumor growth. |
| Confidence score: |
0.8267 |