| Expression pattern: |
UP |
| Associated gene: |
TWIST1 mRNA, TWIST1 |
| Associated microRNA: |
miR-409-3p |
| Biological function: |
Promotes malignant phenotypes and gefitinib resistance of NSCLC cells; knockdown restrains cell viability, reduces proliferation, promotes apoptosis, and lowers gefitinib IC50. |
| Molecular mechanism: |
circ_0001658 acts through a ceRNA-like mechanism by directly targeting miR-409-3p, negatively modulating miR-409-3p, and indirectly up-regulating TWIST1 expression to enhance gefitinib resistance. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); drug resistance (promotes); ceRNA regulation (other) |
| Detected method: |
Q
M
|
| Validation methods: |
RT-qPCR; Microarray; Clinical Sample Validation; Transfection; CCK8; BrdU; Annexin V/PI Flow Cytometry; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Western Blot; Bioinformatics Analysis |
| Clinical significance: |
High circ_0001658 expression is associated with TNM stage and differentiation degree of NSCLC tissues and adverse pathological characteristics. |
| Description: |
circ_0001658 is up-regulated in NSCLC tissues and gefitinib-resistant NSCLC cells. It promotes NSCLC malignant phenotypes and gefitinib resistance by sponging miR-409-3p and increasing TWIST1 expression; its knockdown reduces gefitinib IC50, suppresses proliferation, and promotes apoptosis. |
| Confidence score: |
0.7283 |