| Expression pattern: |
UP |
| Associated gene: |
RPS27A, RPL11, c-Myc, MST1, QKI, URH49 |
| Associated microRNA: |
miR-296-3p, miR-17-5p |
| Biological function: |
promotes proliferation, migration and invasion; promotes metastasis |
| Molecular mechanism: |
acts as a protein scaffold forming a ternary complex with RPS27A and RPL11, modulating RPL11 nuclear availability to regulate c-Myc-mediated repression of MST1; circAKT3 biogenesis regulated by flanking Alu elements and QKI; nuclear export regulated by URH49 |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); cell cycle (promotes); other pathway/process (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
circRNA-seq; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Transfection; RNA Pull-Down; RIP (RNA Immunoprecipitation); Co-IP; ChIP / ChIP-seq; Western Blot; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Bioinformatics Analysis |
| Clinical significance: |
CircAKT3 was significantly correlated with clinical indicators of disease severity, including D'Amico risk classification, the Gleason score, and pT stage. |
| Description: |
circAKT3 is up-regulated in prostate cancer and promotes proliferation, migration, invasion and metastasis. It acts mainly as a protein scaffold that strengthens RPS27A-RPL11 binding and reduces nuclear RPL11, thereby relieving RPL11 inhibition of c-Myc and enhancing c-Myc-mediated repression of MST1. |
| Confidence score: |
0.8621 |