circRNA basic information
circBase ID: -
Name: hsa_circ_ACC1
Synonym: hsa_circ_001391
Host Gene: ACC1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer / CRC
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

colorectal cancer tissues; adjacent non-cancerous colon tissues; cancer and adjacent non-cancerous colon tissues
Cell lines:

HCT116; LO2; 293T; HeLa; A549; HepG2; HT29; U2OS; H1299; MCF7; Hep3B; HAFF
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: AMPK b1, AMPK g1, AMPK holoenzyme, c-Jun, AMPK, ACC1, PFKFB3
Associated microRNA: -
Biological function: circACC1 promotes AMPK activity, glycolysis, fatty acid oxidation, cancer cell proliferation, clonogenic survival, and tumor xenograft growth; circACC1 depletion increases lipid accumulation and retards tumor growth.
Molecular mechanism: circACC1 is induced by c-Jun under serum deprivation and directly binds AMPK b1 and g1 subunits to facilitate AMPK holoenzyme assembly, stability, and activation, thereby promoting AMPK-dependent metabolic reprogramming including fatty acid oxidation and glycolysis.
Biological pathway or process:

glycolysis (promotes); lipid metabolism (other); proliferation (promotes); other pathway/process (promotes); ubiquitination (inhibits)
Detected method:
Q
B
H
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; Northern Blot; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Co-IP; ChIP / ChIP-seq; Luciferase Reporter Assay; Transfection; Colony Formation Assay; In Vivo Animal Model; Western Blot; Bioinformatics Analysis
Clinical significance:

circACC1 expression is elevated in the majority of colorectal cancers and positively correlates with activated c-Jun, AMPK activation, and AMPK subunit levels in cancer tissues.
Description:

circACC1 is a human ACC1-derived circular RNA that is up-regulated in most colorectal cancer tissues examined. It is induced by c-Jun under serum deprivation and directly binds AMPK b1 and g1 subunits to promote AMPK holoenzyme assembly, stability, and activation. Through AMPK-dependent metabolic reprogramming, circACC1 promotes glycolysis, fatty acid oxidation, cancer cell proliferation, and xenograft tumor growth.
Confidence score:

0.8621
Other information
Title:

CircACC1 Regulates Assembly and Activation of AMPK Complex under Metabolic Stress.

Journal: Cell metabolism
Published: 2019
PubMed ID: 31155494
Study type:

combined biological and clinical study
Data availability: https://doi.org/10.17632/79kfhcvbmt.1
Code availability: -