| Expression pattern: |
DN |
| Associated gene: |
PTEN |
| Associated microRNA: |
miR-494 |
| Biological function: |
Prognostic biomarker; high expression is associated with improved outcome; included in multivariate Cox regression RNA signature for CSS/RFS/OS. |
| Molecular mechanism: |
In-silico suggested miR-494 sponging affecting PTEN (loss of sponging when circRHOBTB3 is down-regulated); not experimentally confirmed. |
| Biological pathway or process: |
ceRNA regulation (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RNase R Treatment; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Clinical Sample Validation; Cohort Study; Survival Analysis; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
Associated with cancer-specific survival (high expression improves outcome) and retained as an independent prognostic factor in multivariate Cox models; used in an RNA signature that improves prognostic accuracy when combined with clinicopathological variables. |
| Description: |
CircRHOBTB3 is down-regulated in ccRCC tumor tissue versus adjacent normal tissue. Higher circRHOBTB3 expression predicts better survival and it remains in multivariate prognostic RNA signatures; mechanistically, in-silico analysis suggests a possible circRHOBTB3/miR-494/PTEN ceRNA link, but this was not experimentally validated. |
| Confidence score: |
0.7278 |