| Expression pattern: |
DN |
| Associated gene: |
MIA2 |
| Associated microRNA: |
miR-1200 |
| Biological function: |
Suppresses osteosarcoma cell proliferation, migration, invasion, and xenograft growth. |
| Molecular mechanism: |
circNRIP1 functions as a miRNA sponge for miR-1200, thereby upregulating the miR-1200 target gene MIA2 and inhibiting osteosarcoma progression. |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); invasion (inhibits); ceRNA regulation (promotes); metastasis (inhibits) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Microarray; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis |
| Clinical significance: |
Potential novel approach to control osteosarcoma progression clinically in the future. |
| Description: |
circNRIP1 (hsa_circ_0004771), derived from NRIP1, is downregulated in osteosarcoma cell lines and mainly localized in the cytoplasm. Its overexpression suppresses osteosarcoma proliferation, migration, invasion, and xenograft growth by sponging miR-1200 and upregulating the miR-1200 target gene MIA2. |
| Confidence score: |
0.8109 |