| Expression pattern: |
UP |
| Associated gene: |
PGK1, PTBP1, AGO2 |
| Associated microRNA: |
miR-185-3p |
| Biological function: |
Promotes glycolysis, proliferation, migration, invasion and tumorigenesis in gastric cancer; enhances PGK1 expression and mRNA stability. |
| Molecular mechanism: |
Acts as a ceRNA sponging miR-185-3p to regulate PGK1; binds the RBP PTBP1 and promotes PTBP1 cytoplasmic export, enhancing PTBP1 binding to PGK1 3'UTR and stabilizing PGK1 mRNA. |
| Biological pathway or process: |
glycolysis (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (promotes); mRNA stability (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High hsa_circ_0001756 expression is associated with tumor size and T and N stage, and indicates worse prognosis in GC patients. |
| Description: |
In gastric cancer, hsa_circ_0001756 is up-regulated and promotes glycolysis and malignant phenotypes (proliferation, migration/invasion) and tumor growth. Mechanistically, it sponges miR-185-3p to increase PGK1 and also binds PTBP1 to enhance PTBP1 cytoplasmic localization and stabilize PGK1 mRNA, jointly elevating PGK1-driven glycolysis; high expression predicts poorer patient prognosis. |
| Confidence score: |
0.8913 |