circRNA basic information
circBase ID: hsa_circ_0001649
Name: hsa_circ_SHPRH
Synonym: hsa_circ_001599
Host Gene: SHPRH
Genomic location(hg19): chr6:146209155-146216113:-
Genomic location(hg38): chr6:145888019-145894977:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma / HCC
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

HCC tissues; paired adjacent liver tissues; paired paracancerous liver tissue
Cell lines:

HCC-LM3; MHCC-97L
In vivo animal model:

-
circRNA-disease information
Expression pattern:
DN
Associated gene: MMP9, MMP10, MMP13, U2AF65, EIF4A3, UPF1
Associated microRNA: hsa-miR-1283, hsa-miR-4310, hsa-miR-182-3p, hsa-miR-888-3p, hsa-miR-4502, hsa-miR-6811-5p, hsa-miR-6511b-5p, hsa-miR-1972
Biological function: hsa_circ_0001649 is downregulated in HCC tissues, may serve as a biomarker for HCC, and its low expression is associated with larger tumor size, tumor embolus, metastasis-related MMP expression, poor prognosis, tumorigenesis and metastasis.
Molecular mechanism: Potential protein sponge or transcription regulator involving predicted RBP binding sites, potential interaction with miRNAs, and regulation of metastasis-associated MMP mRNA levels after hsa_circ_0001649 knockdown.
Biological pathway or process:

metastasis (inhibits); invasion (inhibits); proliferation (inhibits); other pathway/process (other)
Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RT-qPCR; Clinical Sample Validation; Transfection; ROC Analysis; Bioinformatics Analysis
Clinical significance:

hsa_circ_0001649 may serve as a novel potential biomarker for HCC; expression was correlated with tumor size and tumor embolus, with ROC AUC of 0.63, cutoff value 0.0007855, sensitivity 0.81, and specificity 0.69; downregulation is associated with poor prognosis of HCC.
Description:

This study found that hsa_circ_0001649, a SHPRH-derived circRNA, is significantly downregulated in HCC tissues compared with paired adjacent liver tissues. Low hsa_circ_0001649 expression was associated with tumor size, tumor embolus, metastasis-related MMP expression, and poor prognosis, suggesting a potential tumor-suppressive role in HCC invasion/metastasis and value as a diagnostic biomarker.
Confidence score:

0.6846
Other information
Title:

Hsa_circ_0001649: A circular RNA and potential novel biomarker for hepatocellular carcinoma.

Journal: Cancer biomarkers : section A of Disease markers
Published: 2016
PubMed ID: 26600397
Study type:

combined biological and clinical study
Data availability: -
Code availability: -