| Expression pattern: |
UP |
| Associated gene: |
PDL1 |
| Associated microRNA: |
miR-511-3p |
| Biological function: |
Promotes NSCLC cell viability, proliferation, invasion, EMT, angiogenesis, immune escape, and xenograft tumor growth; inhibits apoptosis. |
| Molecular mechanism: |
circ_0003528 functions as a cytoplasmic miR-511-3p sponge and increases PDL1 expression, thereby promoting malignant transformation and immune escape in NSCLC. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); invasion (promotes); EMT (promotes); angiogenesis (promotes); immune regulation (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; MTT; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Tube Formation Assay; ELISA; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circ_0003528 expression was associated with worse prognosis and shorter 5-year overall survival in NSCLC patients; it may serve as a therapeutic target for NSCLC. |
| Description: |
In this study, circ_0003528 was up-regulated in NSCLC tissues and cell lines and high expression predicted worse prognosis. Functionally, circ_0003528 promoted malignant transformation, angiogenesis, immune escape, and xenograft tumor growth while suppressing apoptosis. Mechanistically, it acted as a cytoplasmic sponge for miR-511-3p to increase PDL1 expression, forming a circ_0003528/miR-511-3p/PDL1 regulatory axis. |
| Confidence score: |
0.8354 |