circRNA basic information
circBase ID: hsa_circ_0060967
Name: hsa_circ_RAE1
Synonym: circ_0060967
Host Gene: RAE1
Genomic location(hg19): chr20:55931552-55943868:+
Genomic location(hg38): chr20:57356496-57368812:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer / CRC
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

CRC tissue specimens; paired healthy specimens; adjacent normal tissues; xenograft tumors
Cell lines:

HCT-116; SW480; HCO
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: SRC, Ago2, NF-kappaB pathway, p65, IkappaBalpha
Associated microRNA: miR-1184
Biological function: Promotes CRC cell growth, proliferation, colony formation, migration, invasion, motility and tumor growth; suppresses apoptosis.
Molecular mechanism: circ_0060967 functions as a miRNA sponge for miR-1184, thereby up-regulating SRC and activating the SRC/NF-kappaB pathway.
Biological pathway or process:

ceRNA regulation (promotes); NF-kappaB (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); metastasis (promotes)
Detected method:
Q
M
Validation methods:

RT-qPCR; Microarray; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; MTT; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry)
Clinical significance:

circ_0060967 abundance was significantly correlated with tumor size, tumor node metastasis stage, and distant metastasis; it might be implicated in CRC progression and may be a promising target for CRC therapy.
Description:

circ_0060967 is up-regulated in colorectal cancer tissues and CRC cell lines and correlates with tumor size, TNM stage, and distant metastasis. Functionally, circ_0060967 promotes CRC cell proliferation, migration, invasion, and xenograft tumor growth while inhibiting apoptosis. Mechanistically, it sponges miR-1184 to up-regulate SRC and activate the NF-kappaB pathway.
Confidence score:

0.786
Other information
Title:

Circ_0060967 contributes to colorectal cancer progression by sponging miR-1184 to up-regulate SRC proto-oncogene.

Journal: Arab journal of gastroenterology : the official publication of the Pan-Arab Association of Gastroenterology
Published: 2023
PubMed ID: 37045727
Study type:

combined biological and clinical study
Data availability: Supplementary data to this article can be found online at https://doi. org/10.1016/j.ajg.2023.02.001.
Code availability: -