| Expression pattern: |
DN |
| Associated gene: |
Ago2, AKT, mTOR |
| Associated microRNA: |
miR-4712-5p |
| Biological function: |
Promotes colon cancer cell proliferation and migration and inhibits autophagy; hBD-1 reverses these effects. |
| Molecular mechanism: |
Acts as a miR-4712-5p sponge (Ago2-associated), modulating AKT/mTOR phosphorylation to regulate autophagy. |
| Biological pathway or process: |
AKT/mTOR (other); autophagy (inhibits); proliferation (promotes); migration (promotes) |
| Detected method: |
Q
S
M
|
| Validation methods: |
RNA-seq; Microarray; RT-qPCR; RNase R Treatment; divergent primers PCR; RIP (RNA Immunoprecipitation); Transfection; CCK8; Wound Healing Assay; Western Blot |
| Clinical significance: |
has-circpedia-5280/miR-4712-5p can be a diagnostic and prognostic biomarker. |
| Description: |
In SW-620 and HCT-116 colon cancer cells, has-circpedia-5280 (from host gene LRP5) acts as a sponge for miR-4712-5p and modulates AKT/mTOR phosphorylation, thereby inhibiting autophagy and promoting proliferation and migration. hBD-1 treatment decreases has-circpedia-5280 and promotes autophagy, counteracting the effects of has-circpedia-5280 overexpression. |
| Confidence score: |
0.7094 |