| Expression pattern: |
UP |
| Associated gene: |
SOX10, Nestin, Ago2 |
| Associated microRNA: |
miR‐637 |
| Biological function: |
Promotes glioma stemness, self-renewal/proliferation and glycolysis; inhibits apoptosis; promotes xenograft growth in vivo. |
| Molecular mechanism: |
circEPHB4 sponges miR-637 and releases suppression of SOX10, increasing SOX10 and downstream Nestin; confirmed by luciferase reporter assay and Ago2-RIP. |
| Biological pathway or process: |
stemness (promotes); proliferation (promotes); cell cycle (promotes); apoptosis (inhibits); glycolysis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; MTT; Colony Formation Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Flow Cytometry（Non-apoptosis/cycle）; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model |
| Clinical significance: |
Higher circEPHB4 level was independently correlated with reduced overall survival of glioma patients; high circEPHB4 expression was associated with advanced clinical stage and distant metastasis. |
| Description: |
circEPHB4 is up-regulated in gliomas and promotes glioma stemness, proliferation/cell-cycle progression, and glycolysis while inhibiting apoptosis. Mechanistically, it acts as a ceRNA by sponging miR-637, thereby up-regulating SOX10 and its downstream target Nestin; high circEPHB4 is associated with poorer overall survival. |
| Confidence score: |
0.7794 |