| Expression pattern: |
UP |
| Associated gene: |
NFIB |
| Associated microRNA: |
miR-346 |
| Biological function: |
promotes cell proliferation, migration and invasion; promotes tumor growth in vivo |
| Molecular mechanism: |
ceRNA mechanism: sponges miR-346 to upregulate NFIB |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Sanger Sequencing; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Survival Analysis; ROC Analysis; Transfection; CCK8; Transwell Assay; Luciferase Reporter Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model |
| Clinical significance: |
Upregulated in CRC patient tissues and plasma; AUC of hsa_circ_0026416 (0.767) was greater than CEA, CA19-9 and CA72-4; high expression is an independent prognostic factor and indicates poor prognosis. |
| Description: |
hsa_circ_0026416 is up-regulated in colorectal cancer tissues and plasma and primarily localizes to the cytoplasm. It promotes CRC proliferation, migration and invasion (and tumor growth in xenografts) by sponging miR-346, thereby increasing NFIB expression, and its high expression is associated with poorer overall survival and diagnostic value. |
| Confidence score: |
0.8094 |