| Expression pattern: |
UP |
| Associated gene: |
LEF1, Ago2 |
| Associated microRNA: |
miR-936 |
| Biological function: |
promotes osteosarcoma cell proliferation, migration, invasion, angiogenesis and tumor growth |
| Molecular mechanism: |
ceRNA sponge of miR-936 to regulate LEF1 |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); angiogenesis (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Colony Formation Assay; EdU Staining; Transwell Assay; Wound Healing Assay; Tube Formation Assay; Western Blot; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Clinical Sample Validation |
| Clinical significance: |
circDOCK1 expression was associated with tumor size, clinical stage, and distant metastasis; diagnostic and therapeutic potential for OS treatment |
| Description: |
circDOCK1 is up-regulated in osteosarcoma tissues and cell lines and mainly localizes to the cytoplasm. It promotes osteosarcoma malignant behaviors (proliferation, migration, invasion, angiogenesis and tumor growth) by acting as a ceRNA that sponges miR-936, thereby relieving miR-936-mediated repression of LEF1. |
| Confidence score: |
0.8039 |