circRNA basic information
circBase ID: hsa_circ_0007841
Name: hsa_circ_SEC61A1
Synonym: circ_0007841
Host Gene: SEC61A1
Genomic location(hg19): chr3:127778944-127779504:+
Genomic location(hg38): chr3:128060101-128060661:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer / NSCLC
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tissues; matched paracancerous tissues; adjacent normal tissues; tumor tissues
Cell lines:

NCI-H1299; NCI-H1975; A549; PC9; EBAS-2B
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: SphK2, SEC61A1
Associated microRNA: miR-199a-5p
Biological function: Promotes NSCLC cell viability, proliferation, colony formation, migration, invasion, adhesion-related protein changes, and tumor growth in vivo.
Molecular mechanism: circ_0007841 acts as a miR-199a-5p sponge to increase SphK2 expression, thereby promoting NSCLC progression.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes); other pathway/process (promotes)
Detected method:
Q
Validation methods:

RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Wound Healing Assay; IHC (Immunohistochemistry); IF (Immunofluorescence); Western Blot; In Vivo Animal Model; Bioinformatics Analysis
Clinical significance:

Potential aid for improvement of NSCLC treatments.
Description:

circ_0007841 is up-regulated in NSCLC tissues and cell lines and functions as an oncogenic circRNA. Mechanistically, it sponges miR-199a-5p to increase SphK2 expression, thereby promoting NSCLC cell proliferation, migration, invasion, adhesion-related protein changes, and xenograft tumor growth.
Confidence score:

0.8443
Other information
Title:

Circular RNA circ_0007841 participates in progression of nonsmall cell lung cancer via miR-199a-5p/SphK2 axis.

Journal: Anti-cancer drugs
Published: 2022
PubMed ID: 36066393
Study type:

combined biological and clinical study
Data availability: the datasets used and analyzed during the current study are available from the corresponding author upon reasonable request
Code availability: -