| Expression pattern: |
UP |
| Associated gene: |
IGF1, FGF2 |
| Associated microRNA: |
miR-208a, miR-3164 |
| Biological function: |
Promotes TNBC cell proliferation, colony formation, migration, tumorigenesis and metastasis, and inhibits apoptosis. |
| Molecular mechanism: |
circRAD18 functions as a competing endogenous RNA that sponges miR-208a and miR-3164, relieving repression of IGF1 and FGF2 expression. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); apoptosis (inhibits); metastasis (promotes); ceRNA regulation (other); other pathway/process (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; Microarray; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; Western Blot; In Vivo Animal Model; Cohort Study; Survival Analysis; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
circRAD18 was positively correlated with T stage, clinical stage and pathological grade; high circRAD18 expression was an independent risk factor and associated with poorer overall survival; ROC analysis suggested diagnostic value for differentiating TNBC from normal mammary tissues. |
| Description: |
circRAD18 (hsa_circ_0002453), derived from RAD18, is up-regulated in TNBC tissues and cell lines and is associated with advanced clinicopathologic features and poorer overall survival. Functionally, circRAD18 promotes proliferation, migration, tumor growth and metastasis while suppressing apoptosis. Mechanistically, it acts as a ceRNA sponge for miR-208a and miR-3164, thereby increasing IGF1 and FGF2 expression. |
| Confidence score: |
0.8673 |