| Expression pattern: |
UP |
| Associated gene: |
EZH2, CCND1, cyclin D1, CDK4 |
| Associated microRNA: |
miR-217 |
| Biological function: |
circ100284 promotes arsenite-accelerated G1/S cell-cycle transition and contributes to colony formation, invasion, migration, malignant transformation, and neoplastic/metastatic capacity of arsenite-transformed HaCaT cells. |
| Molecular mechanism: |
circ100284 acts as a miR-217 sponge, relieving miR-217-mediated suppression of EZH2; EZH2 up-regulates cyclin D1 and CDK4 and binds the CCND1 promoter to promote cell-cycle acceleration and malignant transformation. |
| Biological pathway or process: |
cell cycle (promotes); proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; Microarray; Nuclear-Cytoplasmic Fractionation; Bioinformatics Analysis; Transfection; Cell Cycle Assay; Colony Formation Assay; Transwell Assay; Western Blot; ChIP / ChIP-seq |
| Clinical significance: |
- |
| Description: |
circ100284 is up-regulated in arsenite-treated and arsenite-transformed human HaCaT keratinocytes. It functions as a miR-217 sponge to increase EZH2, leading to up-regulation of cyclin D1/CDK4, acceleration of the G1/S cell-cycle transition, and enhanced malignant transformation phenotypes including colony formation, invasion, and migration. |
| Confidence score: |
0.7328 |