| Expression pattern: |
DN |
| Associated gene: |
TET1, Ago2, Twist |
| Associated microRNA: |
miR-767-5p |
| Biological function: |
Suppresses osteosarcoma progression by inhibiting proliferation, migration and invasion in vitro and inhibiting tumour growth in vivo. |
| Molecular mechanism: |
Acts as a ceRNA by binding miR-767-5p, thereby modulating the miR-767-5p target TET1; associated with inhibition of EMT marker Twist. |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); invasion (inhibits); EMT (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Sanger Sequencing; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; ROC Analysis; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; EdU Staining; Transwell Assay; In Vivo Animal Model; Western Blot; IHC (Immunohistochemistry); H&E Staining |
| Clinical significance: |
EVs-encapsulated circ-0000190 could distinguish osteosarcoma patients from controls (ROC AUC 0.889) and lower circ-0000190 expression correlated with bigger tumour size, advanced staging and distant metastasis. |
| Description: |
hsa_circ_0000190 (circ-0000190) is down-regulated in osteosarcoma tissues and plasma EVs and functions as a tumour suppressor. It is transferred via EVs from normal osteoblasts to osteosarcoma cells, where it mainly localizes to the cytoplasm and sponges miR-767-5p to modulate TET1, inhibiting proliferation, migration, invasion and tumour growth, with diagnostic potential (AUC 0.889). |
| Confidence score: |
0.8595 |