| Expression pattern: |
UP |
| Associated gene: |
GPNMB |
| Associated microRNA: |
miR-188-3p |
| Biological function: |
Promotes breast cancer cell proliferation, migration, invasion, tumorigenicity and EMT-related malignant behavior, and inhibits autophagy. |
| Molecular mechanism: |
circ_0065214 acts as a ceRNA/miRNA sponge that adsorbs miR-188-3p, relieving miR-188-3p-mediated repression of GPNMB. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); autophagy (inhibits); cell cycle (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Microarray; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Cell Cycle Assay; Transwell Assay; Wound Healing Assay; Western Blot; RNA-seq; IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
circ_0065214 has potential diagnostic value in breast cancer serum; combined application with CEA and CA-153 can further improve diagnostic efficiency. |
| Description: |
circ_0065214 is an SCAP-derived human circRNA up-regulated in breast cancer tissues, serum and cell lines. It promotes breast cancer proliferation, migration, invasion and tumor growth while inhibiting autophagy through a circ_0065214/miR-188-3p/GPNMB ceRNA axis. Serum circ_0065214 also shows diagnostic potential for breast cancer, especially when combined with CEA and CA-153. |
| Confidence score: |
0.8585 |