| Expression pattern: |
UP |
| Associated gene: |
MAP4K3 |
| Associated microRNA: |
let-7b-5p |
| Biological function: |
Inhibits osteogenic differentiation of PDLSCs under inflammatory conditions and promotes autophagy. |
| Molecular mechanism: |
Acts as a miRNA sponge for let-7b-5p to regulate MAP4K3, thereby modulating autophagy and osteogenic differentiation. |
| Biological pathway or process: |
autophagy (promotes); proliferation (inhibits); apoptosis (promotes); osteogenic differentiation (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Western Blot; Transfection; IF (Immunofluorescence); TEM; In Vivo Animal Model; Flow Cytometry（Non-apoptosis/cycle）; CCK8; Oil Red O Staining |
| Clinical significance: |
circ-CDK8 is upregulated in periodontitis tissues and proposed as a therapeutic target for periodontitis/periodontal regeneration. |
| Description: |
circ-CDK8 is upregulated in periodontitis tissues and functionally suppresses osteogenic differentiation of PDLSCs under inflammatory stimulation by promoting autophagy. It acts via a ceRNA mechanism, sponging let-7b-5p to regulate MAP4K3, and its inhibition enhances osteogenesis and reduces autophagy. |
| Confidence score: |
0.4249 |