| Expression pattern: |
UP |
| Associated gene: |
IGF2BP3, NR1H4 |
| Associated microRNA: |
- |
| Biological function: |
CircRHOBTB3 acts as an oncogenic circRNA in cervical cancer cells, promoting cell proliferation, migration, invasion, and Warburg effects. |
| Molecular mechanism: |
CircRHOBTB3 interacts with the RNA-binding protein IGF2BP3 and stabilizes IGF2BP3 expression; NR1H4 putatively transcriptionally regulates circRHOBTB3 through binding the RHOBTB3 promoter. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); glycolysis (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; EdU Staining; Transwell Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
CircRHOBTB3/hsa_circ_0007444 is up-regulated in cervical cancer cell lines and functions as an oncogenic circRNA. Its knockdown suppresses proliferation, migration, invasion, and Warburg effects, while mechanistically it interacts with IGF2BP3 to stabilize IGF2BP3 expression and is putatively transcriptionally regulated by NR1H4. |
| Confidence score: |
0.7909 |