| Expression pattern: |
UP |
| Associated gene: |
ARRDC3, PALM |
| Associated microRNA: |
miR-378 |
| Biological function: |
FZKA induces hsa_circ_0048091 expression; hsa_circ_0048091 inhibits NSCLC cell viability/proliferation and mediates the inhibitory effect of FZKA on NSCLC cell growth. |
| Molecular mechanism: |
hsa_circ_0048091 functions as a ceRNA sponge for hsa-miR-378g, thereby regulating the miR-378g target ARRDC3 in the FZKA response of NSCLC cells. |
| Biological pathway or process: |
proliferation (inhibits); ceRNA regulation (promotes); apoptosis (promotes); drug resistance (other) |
| Detected method: |
Q
H
S
|
| Validation methods: |
RNase R Treatment; circRNA-seq; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; ISH (In Situ Hybridization); IHC (Immunohistochemistry); Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; MTT; Annexin V/PI Flow Cytometry; In Vivo Animal Model; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
Potential therapeutic target for NSCLC treatment in the context of FZKA; no direct patient diagnostic or prognostic association was reported for hsa_circ_0048091. |
| Description: |
hsa_circ_0048091 is a PALM-derived human circRNA induced by FZKA treatment in NSCLC cells and xenograft tumor tissues. It suppresses NSCLC cell proliferation/viability and mediates the anti-proliferative effect of FZKA by sponging hsa-miR-378g and regulating the downstream target ARRDC3. |
| Confidence score: |
0.8516 |