circRNA basic information
circBase ID: hsa_circ_0072305
Name: hsa_circ_LIFR
Synonym: circLIFR-007
Host Gene: LIFR
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: nucleus and cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

primary tumor tissues; paired liver metastatic tissues; liver tissue; liver metastasis specimens
Cell lines:

MCF-10A; MCF-7; MDA-MB-231; MDA-MB-468; BT-549; SKBR-3; T47D
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: hnRNPA1, YAP, YAP1, SREBF1, SNAI1, TEAD
Associated microRNA: -
Biological function: circLIFR-007 inhibits breast cancer cell proliferation, colony formation, migration, invasion, xenograft tumor growth, and liver metastasis.
Molecular mechanism: circLIFR-007 binds hnRNPA1, promotes hnRNPA1 nuclear-to-cytoplasmic transport, facilitates hnRNPA1 interaction with YAP in the cytoplasm, increases YAP phosphorylation, prevents YAP nuclear transcriptional activity, and suppresses liver metastasis-associated proteins SREBF1 and SNAI1.
Biological pathway or process:

proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); Hippo/YAP (inhibits); other pathway/process (inhibits)
Detected method:
Q
M
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Microarray; FISH / smFISH; IF (Immunofluorescence); Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Co-IP; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis
Clinical significance:

circLIFR-007 may serve as a new biomarker to evaluate the risk of recurrence and metastasis of breast cancer.
Description:

circLIFR-007 is a LIFR-derived circRNA that is down-regulated in breast cancer liver metastases and breast cancer cell lines. It suppresses breast cancer proliferation, migration, invasion, tumor growth, and liver metastasis by binding hnRNPA1, promoting hnRNPA1 nuclear export, increasing YAP phosphorylation, retaining YAP in the cytoplasm, and reducing YAP/TEAD-regulated liver metastasis-associated proteins SREBF1 and SNAI1. The study suggests circLIFR-007 may be a biomarker for evaluating breast cancer recurrence and metastasis risk.
Confidence score:

0.8817
Other information
Title:

circLIFR-007 reduces liver metastasis via promoting hnRNPA1 nuclear export and YAP phosphorylation in breast cancer.

Journal: Cancer letters
Published: 2024
PubMed ID: 38685451
Study type:

combined biological and clinical study
Data availability: Supplementary data; https://doi. org/10.1016/j.canlet.2024.216907
Code availability: -