| Expression pattern: |
UP |
| Associated gene: |
TNF-alpha |
| Associated microRNA: |
miR-138, miR-145, miR-24, miR-620, miR-875 |
| Biological function: |
Regulates TNF-alpha expression and promotes extracellular matrix (ECM) degradation in chondrocytes under mechanical stress; its knockdown suppresses TNF-alpha and increases ECM formation. |
| Molecular mechanism: |
Putative miRNA sponge (decoy) mechanism, highlighting miR-875 as a shared miRNA between circRNA-MSR and TNF-alpha 3′ UTR, suggesting ceRNA-like regulation of TNF-alpha. |
| Biological pathway or process: |
ceRNA regulation (other); inflammation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Bioinformatics Analysis; Transfection; Western Blot; IF (Immunofluorescence) |
| Clinical significance: |
Potential therapeutic target for OA (siRNA knockdown proposed to inhibit ECM degradation). |
| Description: |
In osteoarthritis cartilage, circRNA-MSR (hsa_circ_100226; EPS15-derived) is upregulated in damaged regions and increases under mechanical stress in primary chondrocytes. Loss-of-function via siRNA reduces TNF-alpha and restores ECM markers, and bioinformatics suggests it may act as a miRNA sponge (notably involving miR-875) to modulate TNF-alpha. |
| Confidence score: |
0.4852 |