circRNA basic information
circBase ID: hsa_circ_0033550
Name: hsa_circ_AKT1
Synonym: -
Host Gene: AKT1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0002974
MONDO name: cervical cancer
Disease details: cervical cancer
Disease DO ID:
 4362
Disease MeSH ID:
 -
Disease NCIt ID:
 C9311
Disease ICD11 ID:
 1256072522
Disease OMIM ID:
 603956
Species: Human
Species details: Homo sapiens
Tissue specimen:

CC tissues; adjacent non-cancerous tissues; serum; xenograft tumors
Cell lines:

HeLa; CaSki; SiHa; C33A; 293T; H8
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: AKT1, Ago2, TGF-beta
Associated microRNA: miR-942-5p
Biological function: promotes CC cell proliferation and invasion; promotes EMT; promotes tumor growth in vivo
Molecular mechanism: ceRNA sponging of miR-942-5p to upregulate AKT1; induced by TGF-beta leading to EMT regulation
Biological pathway or process:

proliferation (promotes); invasion (promotes); EMT (promotes); ceRNA regulation (promotes)
Detected method:
Q
M
Validation methods:

Microarray; RNase R Treatment; Sanger Sequencing; Back-Splice Junction PCR / divergent primers PCR; RT-qPCR; Northern Blot; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Western Blot; IF (Immunofluorescence); In Vivo Animal Model; ROC Analysis; Bioinformatics Analysis; Clinical Sample Validation
Clinical significance:

circ-AKT1 may have diagnostic value for CC patients
Description:

circ-AKT1 (circ-0033550) is up-regulated in cervical cancer tissues, serum, and CC cell lines, mainly localized in the cytoplasm. It promotes proliferation, invasion, EMT, and xenograft tumor growth by sponging miR-942-5p to upregulate AKT1, forming the circ-AKT1/miR-942-5p/AKT1 axis; TGF-beta induces circ-AKT1 and AKT1 expression.
Confidence score:

0.8781
Other information
Title:

circRNA-AKT1 Sequesters miR-942-5p to Upregulate AKT1 and Promote Cervical Cancer Progression.

Journal: Molecular therapy. Nucleic acids
Published: 2020
PubMed ID: 32193155
Study type:

combined biological and clinical study
Data availability: https://doi.org/10.1016/j.omtn.2020.01.003
Code availability: -