| Expression pattern: |
DN |
| Associated gene: |
ATG13 |
| Associated microRNA: |
miR-361, miR-26a-1, miR-26a-2, miR-214 |
| Biological function: |
promotes autophagy; inhibits proliferation; suppresses tumor growth |
| Molecular mechanism: |
ceRNA mechanism: circHADHA sponges miR-361 to regulate ATG13 expression and autophagy |
| Biological pathway or process: |
autophagy (promotes); proliferation (inhibits); ceRNA regulation (promotes); other pathway/process (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RNase R Treatment; RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; FISH / smFISH; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; TUNEL; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model |
| Clinical significance: |
CircHADHA potentially serves as a biomarker for screening of precursor colon cancer and a therapeutic target for colon cancer treatment. |
| Description: |
CircHADHA (from host gene HADHA) shows dynamic plasma changes across healthy, colon polyp, and colon cancer groups, and is reduced in colon cancer vs polyp. Mechanistically, circHADHA functions as a miRNA sponge (notably miR-361) to increase ATG13, thereby promoting autophagy and suppressing proliferation and xenograft tumor growth in colon cancer cells. |
| Confidence score: |
0.7954 |