| Expression pattern: |
UP |
| Associated gene: |
MAP3K9 |
| Associated microRNA: |
miR-379-5p |
| Biological function: |
Circ_SIPA1L1 promotes osteosarcoma cell proliferation, colony formation, migration, invasion, EMT, metastasis-related protein changes, and tumor growth in vivo. |
| Molecular mechanism: |
Circ_SIPA1L1 acts as a ceRNA by sponging miR-379-5p, thereby upregulating MAP3K9 to promote osteosarcoma progression. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); ceRNA regulation (other); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Actinomycin D / DRB Stability Assay; RNase R Treatment; Transfection; MTT; Colony Formation Assay; Transwell Assay; Western Blot; Luciferase Reporter Assay; Bioinformatics Analysis; In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
The identification of circ_SIPA1L1/miR-379-5p/MAP3K9 axis provides a novel entry point in OS treatment. |
| Description: |
Circ_SIPA1L1 is upregulated in osteosarcoma tissues and cell lines. It promotes OS proliferation, migration, invasion, EMT, and xenograft tumor growth by sponging miR-379-5p and increasing MAP3K9 expression, suggesting the circ_SIPA1L1/miR-379-5p/MAP3K9 axis as a potential therapeutic entry point. |
| Confidence score: |
0.8001 |