circRNA basic information
circBase ID: -
Name: hsa_circ_PRMT5
Synonym: circ-PRMT5
Host Gene: PRMT5
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0001056
MONDO name: gastric cancer
Disease details: gastric cancer / GC
Disease DO ID:
 10534
Disease MeSH ID:
 -
Disease NCIt ID:
 C9331
Disease ICD11 ID:
 1397617262
Disease OMIM ID:
 613659
Species: Human
Species details: Homo sapiens
Tissue specimen:

GC tissues; paired adjacent normal gastric tissues
Cell lines:

AGS; MKN-28; MKN45; BGC823; MGC803; SGC7901; GES-1
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: MYC
Associated microRNA: miR-145, miR-1304
Biological function: Circ-PRMT5 functions as an oncogene in GC; it promotes GC cell growth, clone formation, migration and invasion, promotes EMT, and inhibits apoptosis.
Molecular mechanism: Circ-PRMT5 acts as a ceRNA/miRNA sponge for miR-145 and miR-1304, thereby upregulating MYC expression and promoting gastric cancer progression.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); apoptosis (inhibits)
Detected method:
Q
Validation methods:

RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; Cohort Study; Survival Analysis; Bioinformatics Analysis
Clinical significance:

High circ-PRMT5 expression is associated with poor prognosis, shorter overall survival, larger tumor size, advanced TNM stage, poor differentiation, lymph node metastasis and distant metastasis in GC patients; it may serve as a prognostic biomarker and therapeutic target.
Description:

Circ-PRMT5 is up-regulated in gastric cancer tissues and cell lines and high expression predicts poor overall survival. Functionally, it promotes GC cell proliferation, colony formation, migration, invasion and EMT while inhibiting apoptosis. Mechanistically, circ-PRMT5 sponges miR-145 and miR-1304 to upregulate MYC, forming a circ-PRMT5/miR-145/miR-1304/MYC oncogenic axis.
Confidence score:

0.8143
Other information
Title:

Circ-PRMT5 promotes gastric cancer progression by sponging miR-145 and miR-1304 to upregulate MYC.

Journal: Artificial cells, nanomedicine, and biotechnology
Published: 2019
PubMed ID: 31701767
Study type:

combined biological and clinical study
Data availability: -
Code availability: -