circRNA basic information
circBase ID: hsa_circ_0001666
Name: hsa_circ_FAM120B
Synonym: circular RNA 0001666 / circ_0001666
Host Gene: FAM120B
Genomic location(hg19): chr6:170626457-170639638:+
Genomic location(hg38): chr6:170317369-170330550:+
Subcellular localization: unclear
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer / CRC
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

-
Cell lines:

HT-29; HCT-116; SW480; SW-620; HIEC; 293T
In vivo animal model:

-
circRNA-disease information
Expression pattern:
DN
Associated gene: CXCR5, beta-catenin, GSK3beta, p-GSK3beta
Associated microRNA: miR-1229
Biological function: inhibits cell proliferation, invasion and stemness; promotes apoptosis
Molecular mechanism: circ_0001666 directly binds and negatively regulates miR-1229, thereby inactivating Wnt/beta-catenin signaling (with downstream effects including CXCR5 regulation).
Biological pathway or process:

Wnt/beta-catenin (inhibits); proliferation (inhibits); invasion (inhibits); stemness (inhibits); apoptosis (promotes); ceRNA regulation (other)
Detected method:
Q
Validation methods:

RNase R Treatment; RT-qPCR; Transfection; CCK8; TUNEL; Transwell Assay; Flow Cytometry（Non-apoptosis/cycle）; Luciferase Reporter Assay; Western Blot; FISH / smFISH
Clinical significance:

-
Description:

circ_0001666 is down-regulated in CRC cell lines and functions as a tumor suppressor in vitro. It binds miR-1229, reduces miR-1229 levels, and inactivates Wnt/beta-catenin signaling (with effects involving CXCR5), thereby inhibiting proliferation, invasion and stemness and promoting apoptosis.
Confidence score:

0.6494
Other information
Title:

Circular RNA 0001666 inhibits colorectal cancer cell proliferation, invasion and stemness by inactivating the Wnt/beta-catenin signaling pathway and targeting microRNA-1229.

Journal: Oncology letters
Published: 2022
PubMed ID: 35836485
Study type:

biological research
Data availability: The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Code availability: -