circRNA basic information
circBase ID: hsa_circ_0056881
Name: hsa_circ_DPP4
Synonym: circDPP4
Host Gene: DPP4
Genomic location(hg19): chr2:162877090-162929996:-
Genomic location(hg38): chr2:162020580-162073486:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0008315
MONDO name: prostate cancer
Disease details: prostate cancer
Disease DO ID:
10283
Disease MeSH ID:
D011471
Disease NCIt ID:
C7378
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

PC tumor tissues; paired adjacent non-tumor tissues; xenograft tumor tissues

Cell lines:

RWPE-1; PC3; DU145; LNCaP; PC-3M

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: ZIC2, Ki-67
Associated microRNA: miR-564
Biological function: circDPP4 promotes prostate cancer cell proliferation, colony formation, migration, invasion, tumor growth, and docetaxel resistance, while inhibiting apoptosis; silencing circDPP4 suppresses cell progression and enhances docetaxel cytotoxicity.
Molecular mechanism: circDPP4 acts as a miR-564 sponge and regulates ZIC2 expression through a ceRNA regulatory axis.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); chemoresistance (promotes)

Detected method:
Q
Validation methods:

RNase R Treatment; RT-qPCR; Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Bioinformatics Analysis; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; MTT; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Survival Analysis

Clinical significance:

High circDPP4 expression is associated with shorter overall survival and DTX nonresponsiveness; circDPP4 is suggested as a potential biomarker and therapeutic target for PC.

Description:

circDPP4 is up-regulated in prostate cancer tissues and cell lines, with high expression associated with poor overall survival and DTX nonresponsiveness. Functionally, circDPP4 promotes malignant progression and docetaxel resistance, while its knockdown inhibits proliferation, colony formation, migration, invasion, and tumor growth and promotes apoptosis and DTX cytotoxicity. Mechanistically, circDPP4 acts as a cytoplasmic ceRNA sponge for miR-564 to regulate ZIC2 expression.

Confidence score:

0.849

Other information
Title:

Silencing of circDPP4 suppresses cell progression of human prostate cancer and enhances docetaxel cytotoxicity through regulating the miR-564/ZIC2 axis.

Journal: The journal of gene medicine
Published: 2022
PubMed ID: 34904327
Study type:

combined biological and clinical study

Data availability: All data that generated during this study are available from the corresponding author upon reasonable request.
Code availability: -