circRNA basic information
circBase ID: hsa_circ_0078767
Name: -
Synonym: CircRNA_0078767
Host Gene: -
Genomic location(hg19): chr6:170615843-170639638:+
Genomic location(hg38): chr6:170306755-170330550:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0009807
MONDO name: osteosarcoma
Disease details: osteosarcoma / OS
Disease DO ID:
 3347
Disease MeSH ID:
 D012516
Disease NCIt ID:
 C9145
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

osteosarcoma tissues; tumor adjacent tissues; tumor tissues; lung tissues; xenograft tumors
Cell lines:

U2OS; SaOS-2; HOS; hFOB 1.19; HEK293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: CDK14, AGO2
Associated microRNA: miR-330-3p
Biological function: Promotes osteosarcoma cell viability, proliferation, migration, invasion, tumorigenesis, and metastasis; high expression correlates with poor prognosis.
Molecular mechanism: circ_0078767 acts as a ceRNA by sponging miR-330-3p, reducing miR-330-3p expression and relieving miR-330-3p-mediated inhibition of CDK14, thereby increasing CDK14 expression.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes)
Detected method:
Q
M
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RT-qPCR; Microarray; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Wound Healing Assay; Transwell Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining; Survival Analysis; Cohort Study; Bioinformatics Analysis
Clinical significance:

High circ_0078767 expression correlates with tumor size, histological grade, shorter overall survival, and is an independent risk factor for overall survival in osteosarcoma patients.
Description:

circ_0078767 is up-regulated in osteosarcoma tissues and cells and its high expression is associated with worse overall survival. Mechanistically, it functions as a ceRNA that sponges miR-330-3p to increase CDK14 expression, thereby promoting osteosarcoma cell proliferation, migration, invasion, tumorigenesis, and metastasis.
Confidence score:

0.8557
Other information
Title:

CircRNA_0078767 promotes osteosarcoma progression by increasing CDK14 expression through sponging microRNA-330-3p.

Journal: Chemico-biological interactions
Published: 2022
PubMed ID: 35307379
Study type:

combined biological and clinical study
Data availability: GSE23035; GSE65236; manuscript and supplementary materials; https://doi.org/10.1016/j.cbi.2022.109903
Code availability: -