| Expression pattern: |
UP |
| Associated gene: |
SLC7A11 mRNA, SLC7A11 |
| Associated microRNA: |
miR-139-5p |
| Biological function: |
hsa_circ_0001610 reduces the radiosensitivity of breast cancer cells, promotes cell viability, colony formation, invasion and migration after irradiation, reduces apoptosis and G2/M arrest, and counteracts DHA-induced ferroptosis. |
| Molecular mechanism: |
hsa_circ_0001610 acts as a molecular sponge for miR-139-5p, increases SLC7A11 expression, suppresses ferroptosis, and thereby reduces radiosensitivity; DHA downregulates hsa_circ_0001610 and suppresses the hsa_circ_0001610/miR-139-5p/SLC7A11 pathway. |
| Biological pathway or process: |
ferroptosis (inhibits); radioresistance (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); cell cycle (other); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; FISH / smFISH; Bioinformatics Analysis; Luciferase Reporter Assay; RNA Pull-Down; Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Flow Cytometry（Non-apoptosis/cycle）; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining |
| Clinical significance: |
hsa_circ_0001610 was suggested as a potential biomarker and therapeutic target for breast cancer radiosensitivity. |
| Description: |
In this study, hsa_circ_0001610 is upregulated in irradiated breast cancer cells and functions as a radioresistance-associated circRNA. It acts as a miR-139-5p sponge to increase SLC7A11 expression and suppress ferroptosis, while DHA downregulates hsa_circ_0001610 to enhance ferroptosis and radiosensitivity. |
| Confidence score: |
0.8279 |