| Expression pattern: |
UP |
| Associated gene: |
ABCC1 |
| Associated microRNA: |
miR-199a-5p |
| Biological function: |
promotes adriamycin resistance, invasion and tumor growth; promotes EMT progression |
| Molecular mechanism: |
Acts as a ceRNA (miRNA sponge) for miR-199a-5p to positively regulate ABCC1 expression, promoting EMT and ADM resistance. |
| Biological pathway or process: |
migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); drug resistance (promotes); ceRNA regulation (promotes); proliferation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
divergent primers PCR; RNase R Treatment; RT-qPCR; FISH / smFISH; Luciferase Reporter Assay; RNA Pull-Down; Transfection; Transwell Assay; Cell Cycle Assay; Western Blot; In Vivo Animal Model; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circFoxo3 expression is associated with shorter survival in HCC patients. |
| Description: |
circFoxo3 is up-regulated in HCC, particularly in ADM-resistant and metastatic tissues/cell lines, and high expression correlates with worse patient survival. Functionally, circFoxo3 promotes invasion and tumor growth and contributes to adriamycin resistance by sponging miR-199a-5p, thereby increasing ABCC1 and promoting EMT. |
| Confidence score: |
0.8432 |