| Expression pattern: |
UP |
| Associated gene: |
FAK, EIF4A3 |
| Associated microRNA: |
miR-4725-5p |
| Biological function: |
promoted the growth, migration, invasion, metastasis, and EMT of CRC |
| Molecular mechanism: |
circPTGR1 acts as a ceRNA sponge for miR-4725-5p to upregulate FAK, activating FAK/AKT signaling; EIF4A3 promotes circPTGR1 biogenesis and is also a miR-4725-5p target, forming a positive feedback loop. |
| Biological pathway or process: |
FAK/AKT (promotes); PI3K/AKT (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; FISH / smFISH; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; Western Blot; IHC (Immunohistochemistry); CCK8; Colony Formation Assay; Transwell Assay; H&E Staining; In Vivo Animal Model; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
high circPTGR1 expression was associated with poor prognosis and reduced overall survival in CRC patients; potential prognostic biomarker |
| Description: |
circPTGR1 (hsa_circ_0003731) is upregulated in colorectal cancer and promotes CRC proliferation, migration, invasion, EMT and metastasis. It sponges miR-4725-5p to increase FAK and activate the FAK/AKT (PI3K/AKT-related) pathway, and is embedded in a positive feedback loop with EIF4A3 and miR-4725-5p. High circPTGR1 expression correlates with worse overall survival, suggesting prognostic value. |
| Confidence score: |
0.8657 |