| Expression pattern: |
UP |
| Associated gene: |
MAP3K2, Ago2 |
| Associated microRNA: |
miR-200a-3p |
| Biological function: |
Facilitates HBV replication and hepatoma progression; promotes proliferation, clonogenicity, invasion, migration, metastasis and tumor growth; inhibits apoptosis. |
| Molecular mechanism: |
circBACH1 acts as a ceRNA by sponging miR-200a-3p to regulate MAP3K2 expression, thereby promoting HBV replication and hepatoma development. |
| Biological pathway or process: |
viral replication (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits); ceRNA regulation (other); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; ELISA; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Annexin V/PI Flow Cytometry; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); In Vivo Animal Model; Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
circBACH1 might be a potential therapeutic target for HBV-related hepatoma. |
| Description: |
circBACH1 is up-regulated in HCC tissues and HBV-transfected hepatoma cells. It promotes HBV replication and hepatoma progression by sponging miR-200a-3p and increasing MAP3K2 expression, thereby enhancing proliferation, invasion, migration, metastasis and tumor growth while suppressing apoptosis. circBACH1 may represent a therapeutic target for HBV-related hepatoma. |
| Confidence score: |
0.7951 |