| Expression pattern: |
UP |
| Associated gene: |
TET2, AGO2 |
| Associated microRNA: |
miR-211-5p |
| Biological function: |
promotes lipolysis and white adipose browning (thermogenic remodeling) in adipocytes; promotes adipose wasting in vivo |
| Molecular mechanism: |
Acts as a miRNA sponge (ceRNA) for miR-211-5p to relieve repression of TET2; interaction supported by luciferase reporter, RIP (AGO2), and FISH co-localization |
| Biological pathway or process: |
lipid metabolism (promotes); proliferation (not specified); ceRNA regulation (promotes); mitochondrial function (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; Bioinformatics Analysis; RT-qPCR; RNase R Treatment; divergent primers PCR; Sanger Sequencing; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; Western Blot; Oil Red O Staining; ELISA; IF (Immunofluorescence); In Vivo Animal Model; H&E Staining; ROC Analysis; Survival Analysis; Clinical Sample Validation; Cohort Study |
| Clinical significance: |
circAMOTL1 expression positively correlated with weight loss severity and showed diagnostic performance for cachexia (ROC AUC=0.826). |
| Description: |
circAMOTL1 (hsa_circ_0000350) is up-regulated in subcutaneous adipose tissue of cachectic cancer patients and correlates with weight loss severity. It promotes adipocyte lipolysis and WAT browning by sponging miR-211-5p, which relieves repression of TET2, and it exacerbates adipose wasting in a C26 tumor-bearing mouse cachexia model. It also shows diagnostic value (ROC) and high expression is associated with worse overall survival. |
| Confidence score: |
0.8846 |