circRNA basic information
circBase ID: hsa_circ_0000376
Name: -
Synonym: circ_0000376
Host Gene: -
Genomic location(hg19): chr12:11199618-11248400:-
Genomic location(hg38): chr12:11047019-11095801:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer / NSCLC
Disease DO ID:
3908
Disease MeSH ID:
D002289
Disease NCIt ID:
C2926
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tissues; adjacent healthy lung tissues; tumor tissues

Cell lines:

16HBE; H522; A549

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: PDPK1
Associated microRNA: miR-545-3p
Biological function: Promotes NSCLC cell proliferation, migration, invasion and glutaminolysis, and inhibits apoptosis; promotes tumor growth in vivo.
Molecular mechanism: Acts as a miRNA sponge for miR-545-3p to de-repress PDPK1 (ceRNA-like regulation), thereby regulating malignant phenotypes and glutamine metabolism.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); glutaminolysis (promotes); ceRNA regulation (promotes)

Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Clinical Sample Validation; Cohort Study; Bioinformatics Analysis

Clinical significance:

circ_0000376 expression was closely associated with lymph node metastasis, tumor-node-metastasis stage, and tumor size of NSCLC patients.

Description:

circ_0000376 is up-regulated in NSCLC tissues and cell lines and promotes malignant phenotypes and glutamine metabolism. Mechanistically, it binds (sponges) miR-545-3p to increase PDPK1, and circ_0000376 knockdown suppresses xenograft tumor growth.

Confidence score:

0.7892

Other information
Title:

circ_0000376 knockdown suppresses non-small cell lung cancer cell tumor properties by the miR-545-3p/PDPK1 pathway.

Journal: Open medicine (Warsaw, Poland)
Published: 2023
PubMed ID: 36820067
Study type:

combined biological and clinical study

Data availability: The datasets generated and/or analyzed during the current study are available from the corresponding author on reasonable request.
Code availability: -