| Expression pattern: |
UP |
| Associated gene: |
CDK2, Bcl2, Bax |
| Associated microRNA: |
miR-1296 |
| Biological function: |
Promotes NSCLC cell proliferation, accelerates cell cycle progression, and inhibits apoptosis by up-regulating Bcl2 and down-regulating Bax. |
| Molecular mechanism: |
circRACGAP1 acts as a miR-1296 sponge to increase CDK2 expression, thereby promoting proliferation and suppressing apoptosis in NSCLC cells. |
| Biological pathway or process: |
proliferation (promotes); cell cycle (promotes); apoptosis (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; Cell Cycle Assay; TUNEL; Western Blot |
| Clinical significance: |
Potential diagnostic and therapeutic target for NSCLC. |
| Description: |
circRACGAP1 is up-regulated in NSCLC tissues and cell lines. It promotes NSCLC cell proliferation and cell-cycle progression while inhibiting apoptosis by sponging miR-1296 and increasing CDK2 expression, with downstream effects on Bcl2 and Bax. The study suggests circRACGAP1 may be a potential diagnostic and therapeutic target for NSCLC. |
| Confidence score: |
0.6521 |