circRNA basic information
circBase ID: hsa_circ_0008378
Name: hsa_circ_PIAS1
Synonym: CircPIAS1
Host Gene: PIAS1
Genomic location(hg19): chr15:68434283-68439038:+
Genomic location(hg38): chr15:68141945-68146700:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005105
MONDO name: melanoma
Disease details: melanoma
Disease DO ID:
 1909
Disease MeSH ID:
 D008545
Disease NCIt ID:
 C3224
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissues
Cell lines:

A375; B16-F10
In vivo animal model:

syngeneic model
circRNA-disease information
Expression pattern:
UN
Associated gene: PTBP1, STAT1, SLC7A11, GPX4, PI3K, AKT
Associated microRNA: -
Biological function: promotes tumor progression and suppresses immunogenic ferroptosis; 6-MF-mediated suppression of circPIAS1 biogenesis promotes ferroptosis and inhibits melanoma growth
Molecular mechanism: 6-MF suppresses circPIAS1 biogenesis by downregulating the RBP PTBP1; circPIAS1 (via circPIAS1-108aa) inhibits STAT1 phosphorylation and reactivates the SLC7A11/GPX4 axis, affecting ferroptosis
Biological pathway or process:

PI3K/AKT (inhibits); ferroptosis (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; RNA-seq; Western Blot; Bioinformatics Analysis; Transfection; CCK8; EdU Staining; In Vivo Animal Model; IHC (Immunohistochemistry)
Clinical significance:

-
Description:

In melanoma, circPIAS1 is an oncogenic circRNA (via circPIAS1-108aa) that suppresses STAT1 phosphorylation and supports the SLC7A11/GPX4 program to limit immunogenic ferroptosis. This study shows that 6-methoxyflavone inhibits PTBP1 to suppress circPIAS1 biogenesis, and with IFN-gamma enhances ferroptosis-related oxidative stress and antitumor effects.
Confidence score:

0.6093
Other information
Title:

Six-methoxyflavone suppresses CircPIAS1 biogenesis via targeting PTBP1 and, in combination with IFN-gamma, promotes ferroptosis in melanoma.

Journal: Frontiers in pharmacology
Published: 2025
PubMed ID: 41347180
Study type:

combined biological and clinical study
Data availability: PRJCA047102
Code availability: -