| Expression pattern: |
DN |
| Associated gene: |
Bcl-2, Caspase-3, SHPRH-146aa, RUNX1, NFKBIA |
| Associated microRNA: |
- |
| Biological function: |
Inhibits neuroblastoma cell proliferation, migration, and invasion; promotes apoptosis. |
| Molecular mechanism: |
circ-SHPRH induces apoptosis with downregulation of Bcl-2 and upregulation of Caspase-3; its peptide SHPRH-146aa interacts with RUNX1 to increase NFKBIA expression, and NFKBIA knockdown attenuates the pro-apoptotic effect. |
| Biological pathway or process: |
apoptosis (promotes); proliferation (inhibits); migration (inhibits); invasion (inhibits); other pathway/process (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Bioinformatics Analysis; RT-qPCR; Transfection; CCK8; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot; Microarray |
| Clinical significance: |
- |
| Description: |
circ-SHPRH is down-regulated in neuroblastoma samples/cell lines. Forced circ-SHPRH overexpression suppresses neuroblastoma malignant phenotypes and promotes apoptosis, accompanied by decreased Bcl-2 and increased Caspase-3, with a related SHPRH-146aa/RUNX1/NFKBIA regulatory circuit described in the study. |
| Confidence score: |
0.5067 |