| Expression pattern: |
UP |
| Associated gene: |
KIF1B, AGO2, PI3K/AKT signaling pathway |
| Associated microRNA: |
miR-15b-5p |
| Biological function: |
Promotes malignant transformation and aggressive phenotypes (proliferation, migration, invasion), and promotes EMT and stemness in gastric epithelial cells; enhances tumorigenicity in vivo. |
| Molecular mechanism: |
Exosomal circ0000549 acts as a ceRNA sponge for miR-15b-5p, releasing KIF1B to activate PI3K/AKT signaling. |
| Biological pathway or process: |
PI3K/AKT (promotes); EMT (promotes); proliferation (promotes); migration (promotes); invasion (promotes); stemness (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; RT-qPCR; Clinical Sample Validation; ROC Analysis; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
circ0000549 expression was elevated in serum exosomes from GC patients with high nitrite exposure, and serum exosomal circ0000549 showed diagnostic value by ROC analysis (AUC 0.7816). |
| Description: |
Exosomal circ0000549 is up-regulated in MNNG-transformed gastric epithelial cells and in serum exosomes from GC patients with high nitrite exposure. It promotes proliferation, migration/invasion, EMT and stemness, and increases xenograft tumorigenicity by sponging miR-15b-5p to upregulate KIF1B and activate PI3K/AKT signaling. |
| Confidence score: |
0.8607 |