| Expression pattern: |
UP |
| Associated gene: |
HOXD10, ELAVL1, AGO2, ITGB2, MTCL1 |
| Associated microRNA: |
miR-766 |
| Biological function: |
promotes cell proliferation; promotes migration; promotes invasion; promotes tumor growth in vivo |
| Molecular mechanism: |
Acts as a ceRNA by sponging miR-766 to enhance HOXD10 expression; additionally binds the RBP ELAVL1 to mediate pro-oncogenic effects. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; RNA-seq; Bioinformatics Analysis; Clinical Sample Validation; In Vivo Animal Model; Survival Analysis; Western Blot |
| Clinical significance: |
High hsa_circ_0000825 expression was positively associated with advanced clinical stages, lymph node metastasis, and poor survival. |
| Description: |
hsa_circ_0000825 is up-regulated in LSCC tissues and promotes LSCC cell proliferation, migration, invasion, and xenograft tumor growth. It functions mainly in the cytoplasm by sponging miR-766 to enhance HOXD10 expression and by interacting with the RBP ELAVL1 to support pro-oncogenic effects, and its high expression is associated with poor overall survival. |
| Confidence score: |
0.8769 |