| Expression pattern: |
UP |
| Associated gene: |
GPX2, AGO2, YTHDC1 |
| Associated microRNA: |
miR-1236-3p |
| Biological function: |
Promotes HCC cell proliferation, migration, invasion and tumor growth; suppresses apoptosis; promotes recurrence and lung metastasis. |
| Molecular mechanism: |
m6A-modified circNEK11 is exported to the cytoplasm via YTHDC1; circNEK11 acts as a ceRNA sponge for miR-1236-3p to relieve repression of GPX2, increasing GPX2 expression and promoting HCC progression/recurrence. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes); m6A modification (other) |
| Detected method: |
Q
S
|
| Validation methods: |
circRNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; MeRIP / MeRIP-seq; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis |
| Clinical significance: |
Potential biomarker and therapeutic target for HCC diagnosis and treatment; overexpressed especially in recurrent HCC tissues. |
| Description: |
circNEK11 (hsa_circ_0067347), derived from NEK11 exons 12-15, is up-regulated in HCC (especially recurrent tissues) and promotes malignant phenotypes and tumor growth/metastasis. It acts as a cytoplasmic ceRNA by sponging miR-1236-3p to increase GPX2, and its m6A-dependent nuclear export is facilitated by the reader YTHDC1. |
| Confidence score: |
0.8621 |